Microscopy and microanalysis facility university of new brunswick. Leica confocal sp2 system, the system used can not be properly tested. With the las x control software, leica microsystems offers an interface, which shows all you need to know about your confocal on one screen. Free microscope software zen lite from zeiss microscopy. Leica confocal software, use the book as a tutorial. How to use leica confocal microscope sp5 external laser. Leica tcs sp2 laser scanning spectral confocal microscope part 1 duration.
Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed. It is recommended setting up a separate user id for each user system administrator. Do you struggle with a variety of instruments, protocols, and software interfaces in your lab. Instructions for leica sp2 confocal equipped with visible. Confocal microscopes confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. Thats about it on recovering from leica confocal crashes. Since the lcs software is based on the user administration of windows.
Leica microsystems has developed different types of objective lenses. Leica sp2 aobs confocal microscope microscopes, imagers. Now you know it all, except if the leica lcs software locks up. Use windex and cotton balls or kimwipes to clean coverslips. Configuration six laser excitation which produce the following excitation. Leica tcs sp2 confocal microscope medical and health sciences. Leica sp2aobs confocal microscope revised 100108 the leica sp2 confocal microscope is controlled via software lcs leica confocal software. Leica confocal 2 is installed with t he s23 condenser. The sp2 is a sensitive, flexible confocal for imaging fixed samples. Details the leica tcssp2 has a system magnification of 3. Leica application suite x las x is the one software platform for all leica microscopes.
Basic training video for use of the leica confocal microscope at tufts. The leica sp2 confocal microscope is controlled via software lcs leica confocal software. This will create individual directories that can be viewed only by the respective user. Guaranteed agains doa leica confocal sp2 system, the system used can not be properly tested. Leica tcs sp2 laser scanning spectral confocal microscope part 1. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. The laser light emitted from the leica tcs sp2 used in. Lcs leica confocal software has a fully operator configurable user interface. Please contact your sales person with possible substitutions. Reserve the leica sp2 aobs confocal microscope read the user manual. Free viewer software, lcs lite, available for download from leica ftp site. If this is the case then use ctrlshiftesc hit the process tab, select the lcs software and hit the end process button. The recent innovation from leica microsystems is a new lens dedicated to the work with glycerolwater or similarly embedded specimen. Sp2 confocal this leica sp2 platform is equipped with two leica confocal microscopes.
Leica tcs 4d first fourdetector confocal for multidye applications first spectral detector in confocal microscopy leica tcs sp2 first confocal with excitation from uv to ir hc optics system ensures superlative optical performance first use of aotf for laser modulation in a confocal first dedicated objectives for confocal scanning cs. Open source free software for a leica tcs sp5 confocal. Confocal microscopy list leica sp2 control via labview. On initialization the lcs software tends to report that the scsi system can not be initialized. With the use of the multiphoton laser the microscope system becomes inherently confocal also allowing deeper penetration into tissue with less photobleaching. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems. Leica sp2 confocal microscope institute of biotechnology. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions. The sample stays intact, and the slicing may be repeated many times. The leica dmire2 is the ideal combination of motorization, automation, high stability, optimized system compatibility, ergonomics and user. Leica sp8 confocal with white light laser system operates from a dmi8 cs bino inverted microscope with afc closed loop focusing, scanning and super zgalvo stages, turret cooling, spill protection, and cuda workstation motorized dic accessories. Hi all, i wonder whether anybody has tried succeeded to control a leica sp2 confocal with labview or a software other than the leica software. Standard confocal microscopy with slides and nonstandard specimens, multiphoton microscopy and fluorescence lifetime imaging. This book also covers the way your leica confocal software and the leica microscope interact.
This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Leica pioneered userguiding software in 1995 by introducing workflow arrows to guide the user step by step through the process of sensible data recording. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Imaging uv and vis fluorophores in fixed or live samples. This video shows how to configure the internal laser power wavelength etc. The leica tcs sp2 is supplied with the latest version of the licensed leica confocal software. True confocal scanning tcs is a technique, where only a single, diffraction limited spot is illuminated and observed at a time. Plan in advance which lasers you are going to use before you go to the confocal microscope. Leica tcs sp2 confocal microscopes products home leica leica microsystems. Three different help levels are available for the leica tcs sp2. Fan on turn laser power level to lowest setting min turn argon laser key to start briefly, and then back to on turn green and red hene lasers on with keys if needed. Leica tcssp8 confocal lsm university of new brunswick.
Open source free software for a leica tcs sp5 confocal microscope. Please power this unit up as needed and down when finished. Enter you name, the date, the time, and the account number in the user log book. Make sure that your sample slides are clean and sealed. Leica confocal tcssp2 this is part of the backprojected pinhole calculator. Sp8 lightning confocal microscopes products home leica leica microsystems. Confocal laser scanning microscopy clsm is one of a series of methods to generate slices from microscopic samples by means of optics. Leica sp2 confocal microscope part 4 optics duration. Leica confocal software lcs complements the optics and electronics by.
When you let the mouse pointer hover over a leica confocal software button, a brief. Zen lite is the perfect software solution if you are looking for a viewer to open shared microscope images in czi file format. However, the side length of its square pinhole is usually specified in airy disk units, referenced to a standard fixed wavelength of 580 nm, making it independent of the actual overall magnification. Leica tcs sp2 laser scanning spectral confocal microscope part 1 mikeselectricstuff. Used and remanufactured industrial microscopes for sale. Zeiss lsm 510 confocal inverted microscope system view this machine leica confocal sp2 system view this machine veeco dimension d3100 afm view this machine olympus vs120 100slide scanner with autoloader and basic software view this machine. Free viewer software, lcs lite, available for download from leica ftp site complete offline version available in biru computer room for postprocessing other software packages available within the biru for processing confocal datasets. Leica sp2 confocal microscope part 4 optics youtube. However, for example, some subcellular structures of interest may be beyond abbes diffraction limit and. A higher numerical aperture condenser is used in the leica sp2 confocal 1. Leica tcs sp2 laser scanning confocal microscope operation. On the leica sp2 lcs confocal software scanned images are initially stored in ram and are listed in the experiments box. When the save button is pressed for the first time in a new experiment a save as dialogue box opens requesting for a database name at the box labeled file name.
Leica dmi8 advanced inverted light microscope with motorized stage. Dic optics, high na water and oil immersion objective lenses. National chiao tung university, bio photonics lab, bypraveen kumar. Bellow is a brief stepbystep guide to help new users get started smoothly. Introduction to confocal laser scanning microscopy leica. Also see zeiss lsm780, leica sp8, nikon spinning disk confocal, api omx thin samples, tissue culture and sp5 2photon thick tissue for more live cell imaging options. The image data will be saved to the hard drive once the save. Leica sp2 confocal the uv aotf cooling fan bearing is failing. The leica confocal microscope system b46 weill hall revised65 startup turn on 3 red buttons on far right. Leica confocal sp2 mpflim advanced light microscopy and. Leica tcs sp2 laser scanning spectral confocal microscope. Leica sp2 confocal microscope part 3 electronics youtube. From your confocal image the leica tcs spes streamlined approach makes it accessible to every user, beginners and experts alike.
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